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Oreochromis niloticus considered one of the most important cultured fish species, it is easily cultured, tasty and preferred by many consumers so it is marketed all over the world. Diseases outbreaks were observed worldwide in cultured tilapia specially in the few past years. Current book deal with standard method for induction of experimental infection with bacterial pathogens, standard method for treatment application and some other laboratory studies including serum chemistry and histopathology for determination of treatment success.