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This volume is focused on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. These catalysts have an extensive catalytic repertoire and play pivotal roles in biology where they catalyze chemical transformations in both biosynthetic and degradative pathways. Detailed articles regarding how to study the structures and mechanisms of mononuclear non-heme iron dependent enzymesGuides to the use of partner proteins in non-heme iron enzyme catalysisStrategies to employ mononuclear non-heme iron enzymes in biocatalytic applications