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This volume of "Methods in Enzymology" is the second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. This title is written by expert authors who are leaders in the field. It is extensively referenced and includes useful figures and tables. It provides hints and tricks to facilitate reproduction of methods.