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Cryptic Enzymes and Moonlighting, a new volume in the Foundations and Frontiers in Enzymology series, offers a thorough overview of cryptic enzymes and moonlighting proteins in signalling cascades. It explores recent advances in research, and brings together an array of information across different fields to enable better targeting of these exciting enzymes and their interactomes. With a clear focus on the role of moonlighting and cryptic enzymes in signal transduction, the book considers examples of cryptic enzymes across species, as well in human healthy biology and pathogenesis. In early chapters, leading international contributors discuss evolutionary considerations for moonlighting proteins, moonlighting interactions in the extracellular matrix, eukaryotic moonlighting proteins, modulating, moonlighting kinases, moonlighting proteins in neurobiology signalling, metabolic enzyme moonlighting, and RNA binding and regulatory proteins. Later, methods-driven chapters discuss practical aspects of identifying hidden moonlighting domains in proteins, computational approaches and bioinformatic tools for the identification of cryptic enzymes, establishing cryptic enzyme interactomes, and assessing contributions of moonlighting proteins to signal cascades. Covers recent advances in our understanding of cryptic moonlighting proteins in signal cascades, highlighting and examining key themes across disciplinesEmpowers researchers to better target cryptic enzymes and moonlighting proteins and their interactomes across new research and applicationsFeatures chapter contributions from international leaders in the field